The Use of Sep - Pap C , , Cartridges During the Isolation of Gangliosides Marcia
نویسنده
چکیده
The use of Sep-Pak"! C, , reverse-phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform-methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non-lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroformmethanol.
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